Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen.
Identifieur interne : 000221 ( Main/Exploration ); précédent : 000220; suivant : 000222Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen.
Auteurs : Melis Kucukoglu [Suède, Finlande] ; Salma Chaabouni [Suède] ; Bo Zheng [République populaire de Chine] ; Ari Pekka M Hönen [Finlande] ; Yk Helariutta [Finlande, Royaume-Uni] ; Ove Nilsson [Suède]Source :
- The New phytologist [ 1469-8137 ] ; 2020.
Abstract
The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)-RELATED (CLE) peptide ligands in connection with their receptors are important players in cell-to-cell communications in plants. Here, we investigated the function of the Populus CLV3/ESR-RELATED 47 (PttCLE47) gene during secondary growth and wood formation in hybrid aspen (Populus tremula × tremuloides) using an RNA interference (RNAi) approach. Expression of PttCLE47 peaks in the vascular cambium. Silencing of the PttCLE47 gene expression affected lateral expansion of stems and decreased apical height growth and leaf size. In particular, PttCLE47 RNAi trees exhibited a narrower secondary xylem zone with less xylem cells/cell file. The reduced radial growth phenotype also correlated with a reduced number of cambial cell layers. In agreement with these results, expression of several cambial regulator genes was downregulated in the stems of the transgenic trees in comparison with controls. Altogether, these results suggest that the PttCLE47 gene is a major positive regulator of cambial activity in hybrid aspen, mainly promoting the production of secondary xylem. Furthermore, in contrast to previously characterized CLE genes expressed in the wood-forming zone, PttCLE47 appears to be active at its site of expression.
DOI: 10.1111/nph.16331
PubMed: 31749215
PubMed Central: PMC7065007
Affiliations:
- Finlande, Royaume-Uni, République populaire de Chine, Suède
- Angleterre, Angleterre de l'Est, Uusimaa
- Cambridge, Helsinki
- Université d'Helsinki, Université de Cambridge
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last="Kucukoglu">Melis Kucukoglu</name><affiliation wicri:level="1"><nlm:affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Chaabouni, Salma" sort="Chaabouni, Salma" uniqKey="Chaabouni S" first="Salma" last="Chaabouni">Salma Chaabouni</name><affiliation wicri:level="1"><nlm:affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo" sort="Zheng, Bo" uniqKey="Zheng B" first="Bo" last="Zheng">Bo Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="M Honen, Ari Pekka" sort="M Honen, Ari Pekka" uniqKey="M Honen A" first="Ari Pekka" last="M Hönen">Ari Pekka M Hönen</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Helariutta, Yk" sort="Helariutta, Yk" uniqKey="Helariutta Y" first="Yk" last="Helariutta">Yk Helariutta</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Sainsbury Laboratory, University of Cambridge, CB2 1LR, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Sainsbury Laboratory, University of Cambridge, CB2 1LR, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Nilsson, Ove" sort="Nilsson, Ove" uniqKey="Nilsson O" first="Ove" last="Nilsson">Ove Nilsson</name><affiliation wicri:level="1"><nlm:affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)-RELATED (CLE) peptide ligands in connection with their receptors are important players in cell-to-cell communications in plants. Here, we investigated the function of the Populus CLV3/ESR-RELATED 47 (PttCLE47) gene during secondary growth and wood formation in hybrid aspen (Populus tremula × tremuloides) using an RNA interference (RNAi) approach. Expression of PttCLE47 peaks in the vascular cambium. Silencing of the PttCLE47 gene expression affected lateral expansion of stems and decreased apical height growth and leaf size. In particular, PttCLE47 RNAi trees exhibited a narrower secondary xylem zone with less xylem cells/cell file. The reduced radial growth phenotype also correlated with a reduced number of cambial cell layers. In agreement with these results, expression of several cambial regulator genes was downregulated in the stems of the transgenic trees in comparison with controls. Altogether, these results suggest that the PttCLE47 gene is a major positive regulator of cambial activity in hybrid aspen, mainly promoting the production of secondary xylem. Furthermore, in contrast to previously characterized CLE genes expressed in the wood-forming zone, PttCLE47 appears to be active at its site of expression.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31749215</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>226</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>04</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen.</ArticleTitle><Pagination><MedlinePgn>75-85</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.16331</ELocationID><Abstract><AbstractText>The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)-RELATED (CLE) peptide ligands in connection with their receptors are important players in cell-to-cell communications in plants. Here, we investigated the function of the Populus CLV3/ESR-RELATED 47 (PttCLE47) gene during secondary growth and wood formation in hybrid aspen (Populus tremula × tremuloides) using an RNA interference (RNAi) approach. Expression of PttCLE47 peaks in the vascular cambium. Silencing of the PttCLE47 gene expression affected lateral expansion of stems and decreased apical height growth and leaf size. In particular, PttCLE47 RNAi trees exhibited a narrower secondary xylem zone with less xylem cells/cell file. The reduced radial growth phenotype also correlated with a reduced number of cambial cell layers. In agreement with these results, expression of several cambial regulator genes was downregulated in the stems of the transgenic trees in comparison with controls. Altogether, these results suggest that the PttCLE47 gene is a major positive regulator of cambial activity in hybrid aspen, mainly promoting the production of secondary xylem. Furthermore, in contrast to previously characterized CLE genes expressed in the wood-forming zone, PttCLE47 appears to be active at its site of expression.</AbstractText><CopyrightInformation>© 2019 The Authors New Phytologist © 2019 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kucukoglu</LastName><ForeName>Melis</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-2655-7398</Identifier><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chaabouni</LastName><ForeName>Salma</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Bo</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0002-5337-5477</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mähönen</LastName><ForeName>Ari Pekka</ForeName><Initials>AP</Initials><Identifier Source="ORCID">0000-0001-6051-866X</Identifier><AffiliationInfo><Affiliation>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Helariutta</LastName><ForeName>Ykä</ForeName><Initials>Y</Initials><Identifier Source="ORCID">0000-0002-7287-8459</Identifier><AffiliationInfo><Affiliation>Institute of Biotechnology, Helsinki Institute of Life Science (HILIFE), University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Organismal and Evolutionary Biology Research Programme (OEB), Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Viikki Plant Science Centre, University of Helsinki, 00014, Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Sainsbury Laboratory, University of Cambridge, CB2 1LR, Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nilsson</LastName><ForeName>Ove</ForeName><Initials>O</Initials><Identifier Source="ORCID">0000-0002-1033-1909</Identifier><AffiliationInfo><Affiliation>Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>12</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Populus
</Keyword><Keyword MajorTopicYN="Y">PttCLE47
</Keyword><Keyword MajorTopicYN="Y">CLE peptides</Keyword><Keyword MajorTopicYN="Y">cambium</Keyword><Keyword MajorTopicYN="Y">secondary growth and development</Keyword><Keyword MajorTopicYN="Y">wood formation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>11</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31749215</ArticleId><ArticleId IdType="doi">10.1111/nph.16331</ArticleId><ArticleId IdType="pmc">PMC7065007</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>New Phytol. 2017 Jul;215(2):642-657</Citation><ArticleIdList><ArticleId IdType="pubmed">28609015</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2018 Mar;16(3):808-817</Citation><ArticleIdList><ArticleId IdType="pubmed">28905477</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2011 Jan;75(1-2):67-75</Citation><ArticleIdList><ArticleId IdType="pubmed">21052783</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2000 Jul 28;289(5479):617-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10915624</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Plants. 2018 Dec;4(12):1071-1081</Citation><ArticleIdList><ArticleId IdType="pubmed">30518839</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2018 Apr;556(7700):235-238</Citation><ArticleIdList><ArticleId IdType="pubmed">29618812</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2002 May;14(5):969-77</Citation><ArticleIdList><ArticleId IdType="pubmed">12034890</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2020 Jan;18(1):195-206</Citation><ArticleIdList><ArticleId IdType="pubmed">31199056</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2011 Jan;52(1):37-48</Citation><ArticleIdList><ArticleId IdType="pubmed">20802224</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2013 Sep 9;23(17):1670-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23910659</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2013;4:2191</Citation><ArticleIdList><ArticleId IdType="pubmed">23934307</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2004 Sep;16(9):2278-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15316113</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2003 Feb;51(3):415-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12602871</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2015 Apr 20;25(8):1050-5</Citation><ArticleIdList><ArticleId IdType="pubmed">25866390</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Mar;155(3):1214-25</Citation><ArticleIdList><ArticleId IdType="pubmed">21205615</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2016 Mar 02;17:174</Citation><ArticleIdList><ArticleId IdType="pubmed">26935217</ArticleId></ArticleIdList></Reference><Reference><Citation>Development. 2010 Mar;137(5):767-74</Citation><ArticleIdList><ArticleId IdType="pubmed">20147378</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2008 Jan 03;8:1</Citation><ArticleIdList><ArticleId IdType="pubmed">18171480</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1997 Aug 1;16(15):4738-45</Citation><ArticleIdList><ArticleId IdType="pubmed">9303318</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2011 May;24(5):606-18</Citation><ArticleIdList><ArticleId IdType="pubmed">21198362</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15208-13</Citation><ArticleIdList><ArticleId IdType="pubmed">18812507</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Aug 11;313(5788):842-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16902140</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2006 Feb;140(2):726-33</Citation><ArticleIdList><ArticleId IdType="pubmed">16407446</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2010 Dec;154(4):1721-36</Citation><ArticleIdList><ArticleId IdType="pubmed">20884811</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Jul;55(1):152-60</Citation><ArticleIdList><ArticleId IdType="pubmed">18315543</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2009 Jul;4(7):634-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19820344</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Chem Biol. 2009 Aug;5(8):578-80</Citation><ArticleIdList><ArticleId IdType="pubmed">19525968</ArticleId></ArticleIdList></Reference><Reference><Citation>J Integr Plant Biol. 2019 Oct;61(10):1043-1061</Citation><ArticleIdList><ArticleId IdType="pubmed">31127689</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2010 Aug;22(8):2618-29</Citation><ArticleIdList><ArticleId IdType="pubmed">20729381</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1997 Oct 9;389(6651):553</Citation><ArticleIdList><ArticleId IdType="pubmed">9335491</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):20032-7</Citation><ArticleIdList><ArticleId IdType="pubmed">19064928</ArticleId></ArticleIdList></Reference><Reference><Citation>Development. 2013 May;140(10):2224-34</Citation><ArticleIdList><ArticleId IdType="pubmed">23578929</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2019 Jan;565(7740):485-489</Citation><ArticleIdList><ArticleId IdType="pubmed">30626967</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):2029-34</Citation><ArticleIdList><ArticleId IdType="pubmed">24449877</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2006 Aug;141(4):1284-92</Citation><ArticleIdList><ArticleId IdType="pubmed">16751438</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation><ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11551-6</Citation><ArticleIdList><ArticleId IdType="pubmed">25049386</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2002 May;7(5):193-5</Citation><ArticleIdList><ArticleId IdType="pubmed">11992820</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2019 Mar;42(3):1033-1044</Citation><ArticleIdList><ArticleId IdType="pubmed">30378140</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2017 Jul;29(7):1585-1604</Citation><ArticleIdList><ArticleId IdType="pubmed">28655750</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Sep;17(9):2542-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16055633</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2013 Jul;6(4):1331-43</Citation><ArticleIdList><ArticleId IdType="pubmed">23288865</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2012 Mar;24(3):1000-12</Citation><ArticleIdList><ArticleId IdType="pubmed">22427333</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2008 Jan;3(1):30-3</Citation><ArticleIdList><ArticleId IdType="pubmed">19704763</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2009 Jun 9;19(11):909-14</Citation><ArticleIdList><ArticleId IdType="pubmed">19398337</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2000 Mar 17;100(6):635-44</Citation><ArticleIdList><ArticleId IdType="pubmed">10761929</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1999 Mar 19;283(5409):1911-4</Citation><ArticleIdList><ArticleId IdType="pubmed">10082464</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2009 Jan;50(1):67-77</Citation><ArticleIdList><ArticleId IdType="pubmed">19074184</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2010 May;153(1):222-37</Citation><ArticleIdList><ArticleId IdType="pubmed">20348212</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2012;8(11):e1002997</Citation><ArticleIdList><ArticleId IdType="pubmed">23166504</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18625-30</Citation><ArticleIdList><ArticleId IdType="pubmed">19011104</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Finlande</li><li>Royaume-Uni</li><li>République populaire de Chine</li><li>Suède</li></country><region><li>Angleterre</li><li>Angleterre de 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|texte= Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen.
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